Los componentes del pastoreo afectan de forma diferencial las micorrizas en Paspalum dilatatum Poir.

Los componentes del pastoreo afectan de forma diferencial las micorrizas en Paspalum dilatatum Poir.

Silvina García, Fabiana Pezzani, Felipe Lezama, José M. Paruelo


El pastoreo es una perturbación importante en los ecosistemas de pastizales, ya que altera su estructura y su funcionamiento. Las micorrizas arbusculares son interacciones cosmopolitas de estos ecosistemas, y se destacan por su incidencia elevada en las gramíneas. Las micorrizas muestran respuestas diversas y contradictorias al pastoreo (ser beneficiadas, perjudicadas o no ser afectadas). Una posible hipótesis es que cada componente del pastoreo (defoliación selectiva, deyecciones y pisoteo) diferiría en su importancia relativa al afectar la micorrización. El objetivo del presente trabajo fue evaluar el efecto del pisoteo, la selectividad y la deposición de orina sobre las micorrizas de Paspalum dilatatum, una de las principales gramíneas nativas de los pastizales del Río de la Plata. En un experimento manipulativo en el que se simularon los distintos componentes del pastoreo se evaluó el efecto de cada componente de forma aislada y en interacción sobre la colonización micorrícica total, así como de las diferentes estructuras fúngicas (arbúsculos, ovillos y vesículas). La colonización micorrícica total y por arbúsculos no fueron afectadas por ninguno de los componentes del pastoreo, mientras que sí se observaron efectos sobre las demás estructuras fúngicas. La colonización por ovillos (una de las estructuras que participan en el intercambio con el hospedante) fue afectada de forma positiva por la selectividad y negativa por el agregado de nitrógeno (simulando la deposición de orina); estas estructuras también fueron afectadas por las interacciones selectividad x pisoteo y pisoteo x agregado de N. El pisoteo afectó negativamente la presencia de vesículas, lo que podría deberse, en parte, al daño mecánico que esta perturbación causa tanto sobre las raíces como sobre el micelio extra-radical. El estudio de los componentes del pastoreo de manera aislada mostró que cada uno ejerce efectos diferentes sobre las micorrizas, y su importancia relativa podría afectar el resultado global del pastoreo sobre esta interacción.


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Altamirano, A., H. Da Silva, A. Durán, A. Echeverría, D. Panario, and R. Puentes. 1976. Clasificación de Suelos. Dirección de Suelos y Fertilizantes. Ministerio de Ganadería Agricultura y Pesca. Tomo I. Montevideo, Uruguay.

Altesor, A., E. Di Landro, H. May, and E. Ezcurra. 1998. Long‐term species change in a Uruguayan grassland. Journal of Vegetation Science 9:173-80.

Altesor, A., G. Piñeiro, F. Lezama, R. B. Jackson, M. Sarasola, and J. M. Paruelo. 2006. Ecosystem changes associated with grazing in subhumid South American grasslands. Journal of Vegetation Science 17:323-332.

Altesor, A., E. Leoni, A. Guido, and J. M. Paruelo. 2016. Differential responses of three grasses to defoliation, water and light availability. Plant Ecology 217:95-104.

Ayres, E., J. Heath, M. Possell, H. I. Black, G. Kerstien, and R. D. Bardgett. 2004. Tree physiological responses to above-ground herbivory directly modify below-ground process of soil carbon and nitrogen cycling. Ecology Letters 7:469-479.

Avio, L., M. Castaldini, A. Fabiani, S. Bedini, C. Sbrana, A. Turrini, and M. Giovannetti. 2013. Impact of nitrogen fertilization and soil tillage on arbuscular mycorrhizal fungal communities in a Mediterranean agroecosystem. Soil Biology and Biochemistry 67:285-294.

Bai, G., Y. Bao, G. Du, and Y. Qi. 2013. Arbuscular mycorrhizal fungi associated with vegetation and soil parameters under rest grazing management in a desert steppe ecosystem. Mycorrhiza 23:289-301.

Barto, E. K., and M. C. Rillig. 2010. Does herbivory really suppress mycorrhiza? A meta-analysis. Journal of Ecology 98:745-753.

Bayne, H. G., M. S. Brown, and G. J. Bethlenfalvay. 1984. Defoliation effects on mycorrhizal colonization, nitrogen fixation and photosynthesis in the Glycine-Glomus-Rhizobium symbiosis Physiologia Plantarum 62:576-80.

Bever, J. D., I. Dickie, E. Facelli, J. Facelli, J. Klironomos, M. Moora, M. Rillig, W. Stock, M. Tibbett, and M. Zobel. 2010. Rooting theories of plant community ecology in microbial interactions. Trends in Ecology and Evolution 25:468-78.

Brundrett, M., N. Bougher, B. Dell, T. Grove and N. Malajczvk. 1996. Working with mycorrhizas in forestry and agriculture. Australian Centre for International Agriculture Research. Canberra, Australia.

Bungenstab, E. 2009. Productivity, nutritive quality and utilization of Dallis Grass (Paspalum dilatatum) for beef cattle production as influenced by fertilization regime and grazing management. Tesis de doctorado. Auburn University, Alabama. USA. Pp. 104.

Chaudhry, M. S., M. Saeed, A. A. Khan, N. Sial, and M. Jamil. 2012. Morphological diversity of arbuscular mycorrhiza colonizing two aromatic grasses Veteviria zizanioides and Cymbopogon jwarancusa. Pakistan Journal of Botany 44:1479-1485.

Collins, N., D. Rowland, L. Corkidi, L. Egerton-Warburton, and E. Allen. 2003. Nitrogen enrichment alters mycorrhizal allocation at five mesic to semiarid grasslands. Ecology 84:1895-1908.

Di Rienzo, J., F. Casanoves, M. Balzarini, L. González, M. Tablada, and C. Robledo. 2014. InfoStat versión 2014. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. URL: www.infostat.com.ar.

Dickson, S., F. A. Smith, and S. E. Smith. 2007. Structural differences in arbuscular mycorrhizal symbioses: more than 100 years after Gallaud, where next? Mycorrhiza 17:375-393.

Endresz, G., I. Somodi, and T. Kalapos. 2013. Arbuscular mycorrhizal colonization of roots of grass species differing in invasiveness. Community Ecology 14:67-76.

Eom, A. H., G. W. Wilson, and D. C. Hartnett. 2001. Effects of ungulates grazers on arbuscular mycorrhizal symbiosis and fungal community structure in tallgrass prairie. Mycologia 93:233-242.

García, S., F. Pezzani, A. Rodríguez, and A. del Pino. 2016. Micorrizas en gramíneas nativas: efecto de la fertilización fosfatada a largo plazo. Agrociencia 20:7-16.

Gehring, C., and T. Whitham. 1994. Interactions between aboveground herbivores and the mycorrhizal mutualists of plants. Trends in Ecology and Evolution 9:251-255.

Grigera, G., and M. Oesterheld. 2004. Mycorrhizal colonization patterns under contrasting grazing and topographic conditions in the flooding pampa (Argentina). Journal of Range Management 57:601-605.

Guo, Y., Q. Du, G. Li, Y. Ni, Z. Zhang, W. Ren, and X. Hou. 2016. Soil phosphorus fractions and arbuscular mycorrhizal fungi diversity following long-term grazing exclusion on semi-arid steppes in inner Mongolia. Geoderma 269:79-90.

Hamilton, E. W., D. A. Frank, P. M. Hinchey, and T. R. Murray. 2008. Defoliation induces root exudation and triggers positive rhizospheric feedbacks in a temperate grassland. Soil Biology and Biochemistry 40:2865-2873.

Hamilton, E. W., and D. A. Frank. 2001. Can plants stimulate soil microbes and their own nutrient supply? Evidence from a grazing tolerant grass. Ecology 82:2397-2402.

Hartnett, D., and T. Wilson. 1999. Mycorrhizae influence plant community structure and diversity in tallgrass prairie. Ecology 80:1187-1195.

Hernández, J., O. Otegui, and J. P. Zamalvide. 1995. Formas y contenidos de fósforo en algunos suelos de Uruguay. Boletín de Investigaciones Facultad de Agronomía. Montevideo. Nº 43. Pp. 32.

Hiltbrunner, D., S. Schulze, F. Hagedorn, M. Schmidt, and S. Zimmermann. 2012. Cattle trampling alters soil properties and changes soil microbial communities in a Swiss sub-alpine pasture. Geoderma 170:369-77.

Hodge, A., and K. Storer. 2014. Arbuscular mycorrhiza and nitrogen: implications for individual plants through to ecosystems. Plant Soil 386:1-19.

Hong, J. J., Y. Park, B. Armando, K. Battarai, D. Daniels, and M. J. Harrison. 2012. Diversity of morphology and function in arbuscular mycorrhizal symbioses in Brachypodium distachyon. Planta 236:851-865.

Johnson, N. C., G. W. T. Wilson, M. A. Bowker, J. A. Wilson, and R. M. Miller. 2010. Resource Limitation Is a Driver of Local Adaptation in Mycorrhizal Symbioses. Proceedings of the National Academy of Sciences 107:2093-2098.

Koske, R., and J. Gemma. 1989. A modified procedure for staining roots to detect VA mycorrhizas. Mycological Research 92:486-505.

Lezama, F., and J. M. Paruelo. 2016. Disentangling grazing effects: trampling, defoliation and urine deposition. Applied Vegetation Science 19:557-566.

León, R. 1991. Vegetation. Pp. 380-387 en A. Soriano and R. Coupland (eds.). Natural Grasslands: introduction and Western Hemisphere. Elsevier, Amsterdam, Netherlands.

Li, C. H., B. L. Ma, and T. Q. Zhang. 2002. Soil bulk density effects on soil microbial populations and enzyme activities during the growth of maize (Zea mays L.) planted in large pots under field exposure. Canadian Journal of Soil Science 82:147-154.

Lugo, M., M. González Maza, and M. Cabello. 2003. Arbuscular mycorrhizal fungi in a mountain grassland. II: seasonal variation of colonization studied along with its relation to grazing and metabolic host type. Mycologia 93:407-415.

Ludvíková, V., V. V. Pavlů, J. Gaisler, M. Hejcman, and L. Pavlů. 2014. Long term defoliation by cattle grazing with and without trampling differently affects soil penetration resistance and plant species composition in Agrostis capillaris grassland. Agriculture, Ecosystems and Environment 197:204-11.

Marulanda, A., R. Azcón, and J. Ruiz-Lozano 2003. Contribution of six arbuscular mycorrhizal fungal isolates to water uptake by Lactuca sativa plants under drought stress. Physiologia Plantarum 119:526-533.

McKinley, V. L., A. D. Peacock, and D. C. White. 2005. Microbial community PLFA and PHB responses to ecosystem restoration in tallgrass prairie soils. Soil Biology and Biochemistry 37:1946-1958.

McNaughton, S. J. 1984. Grazing lawns: animals in herds, plant form, and coevolution. The American Naturalist 126:863-886.

McNaughton, S. D., and M. Oesterheld. 1990. Extramatrical mycorrhizal abundance and grass nutrition in a tropical grazing ecosystem the Serengeti National Park, Tanzania. Oikos 59:92-96.

Mendoza, R., V. Goldman, J. Rivas, V. Escudero, E. Pagani, M. Collantes, and L. Marbán. 2002. Poblaciones de hongos micorrícicos arbusculares en relación con las propiedades del suelo y de la planta hospedera en pastizales de Tierra del Fuego. Ecología Austral 12:105-116.

Mendoza, R., M. Cabello, J. Anchorena, I. García, and L. Marbán. 2011. Soil parameters and host plants associated with arbuscular mycorrhizae in the grazed Magellanic steppe of Tierra del Fuego. Agriculture, Ecosystems & Environment 140:411-418.

Parodi, G., and F. Pezzani. 2011. Micorrizas arbusculares en dos gramíneas nativas de Uruguay en áreas con y sin pastoreo. Agrociencia 15:1-10.

Pezzani, F., C. Montaña, and R. Guevara. 2006. Associations between arbuscular mycorrhizal fungi and grasses in the successional context of a two-phase mosaic in the Chihuahuan Desert. Mycorrhiza 16:285-295.

Piippo, S., A. Markkola, E. Härmä, and J. Tuomi. 2011. Do compensatory shoot growth and mycorrhizal symbionts act as competing above and below-ground sinks after simulated grazing? Plant Ecology 212:33-42.

Porras-Alfaro, A., J. Herrera, O. D. Natvig, and R. L. Sinsabaugh. 2007. Effect of long-term nitrogen fertilization on mycorrhizal fungi associated with a dominant grass in a semiarid grassland. Plant and Soil 296:65-75.

Ren, H., W. Gui, Y. Bai, C. Stein, J. L. Rodrigues, G. W. Wilson, A. B. Cobb, Y. Zhang, and G. Yang. 2017. Long-term effects of grazing and topography on extra-radical hyphae of arbuscular mycorrhizal fungi in semi-arid grasslands. Mycorrhiza 28:117-27.

Ritz, K., and I. M. Young. 2004. Interactions between soil structure and fungi. Mycologist 18:52-59.

Ruotsalainen, A., and A. Eskelinen. 2011. Root fungal symbionts interact with mammalian herbivory, soil nutrient availability and specific hábitat conditions. Oecologia 166:807-817.

Saito, K., Y. Suyama, S. Sato, and K. Sugawara. 2004. Defoliation effects on the community structure of arbuscular mycorrhizal fungi based on 18s rADN sequences. Mycorrhiza 14:363-373.

Schausberger, P., S. Peneder, S. Jürschik, and D. Hoffmann. 2012. Mycorrhiza changes plant volatiles to attract spider mite enemies. Functional Ecology 26:441-449.

Smith, S. E., and D. J. Read. 2008. Mycorrhizal Simbiosis. 3rd edition. Academic Press, New York, USA.

Smith, S. E., and F. A. Smith. 2011. Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Annual Review of Plant Biology 62:227-50.

Stillwell, M. A., and R. G. Woodmansee. 1981. Chemical transformation of urea-nitrogen and movement of nitrogen in shortgrass prairie soil. Soil Science Society of America Journal 45:893-898.

Striker, G., P. Insausti, A. Grimoldi, and R. León. 2006. Root strength and trampling tolerance in the grass Paspalum dilatatum and the dicot Lotus glaber in flooded soil. Functional Ecology 20:4-10.

Su, Y., and L. Guo. 2007. Arbuscular mycorrhiza fungi in non-grazed, restored and over-grazed grassland in the Inner Mongolia steppe. Mycorrhiza 17:689-693.

Torres, Y., C. Busso, O. Montenegro, L. Ithurrart, H. Giorgetti, G. Rodríguez, D. Betivenga, R. Brevedan, O. Fernández, M. Mujica, S. Baioni, J. Entío, M. N. Fioreti, and G. Tucat. 2011. Defoliation effects on the arbuscular mycorrhizas of ten perennial grass genotypes in arid Patagonia, Argentina. Applied Soil Ecology 49:208-214.

Tuomi, J., M. M. Kytöviita, and R. Härdling. 2001. Cost efficiency of nutrient acquisition and the advantage of mycorrhizal symbiosis for the host plant. Oikos 92:62-70.

van der Heijden, M., J. Klironomos, M. Ursi, P. Moutoglis, R. Stritwolf-Engel, T. Boller, A. Wiemken, and I. Sanders. 1998. Mycorrhizal fungi diversity determines plant biodiversity, ecosystem variability and productivity. Nature 395:69-72.

van der Heijden, M, and T. R. Horton. 2009. Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems. Journal of Ecology 97:1139-1150.

van der Heyde, M., J. A. Bennett, J. Pither, and M. Hart. 2017. Long term effects of grazing on arbuscular mycorrhizal fungi. Agriculture, Ecosystems and Environment 243:27-33.

Vierheilig, H., A. P. Coughlan, U. Wiss, and Y. Piché. 1998. Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Applied Environmental Microbiology 64:5004-5007.

Walder, F., H. Niemann, M. Natarajan, M. Lehmann, T. Boller, and A. Wiemken. 2012. Mycorrhizal networks: common goods of plants shared under unequal terms of trade. Plant Physiology 159:789-797.

Wallace, L. L. 1981. Growth, morphology and gas exchange of mycorrhizal and nonmycorrhizal Panicum coloratum L., a C4 grass species, under different clipping and fertilization regimes. Oecologia 49:272-278.

Walling, S., and C. A. Zabinski. 2006. Defoliation effects on arbuscular mycorrhizae and plant growth of two native bunchgrasses and an invasive forb. Applied Soil Ecology 32:111-117.

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