Respuesta del perifiton de agua dulce al glifosato grado-técnico y dos formulados comerciales

Respuesta del perifiton de agua dulce al glifosato grado-técnico y dos formulados comerciales

Daniela Gonzalez, Ángela Juárez, C. Pamela Krug, Marina Santos, M. Solange Vera


El glifosato es el herbicida más utilizado a nivel mundial. Puede llegar a los cuerpos de agua y afectar a las comunidades microbianas. Comparamos los efectos del glifosato grado técnico y dos formulados comerciales sobre una comunidad perifítica de agua dulce. Se llevó a cabo un experimento de laboratorio con cuatro tratamientos: glifosato ácido grado técnico, Glifosato II Atanor®, Roundup Max®, a una concentración de 3 mg de ingrediente activo/L, y un Control. A los 2, 5 y 9 días posteriores a la aplicación de los herbicidas se determinaron la concentración de pigmentos, peso seco, peso seco libre de cenizas y densidad algal del perifiton. Se registró un incremento de la abundancia de Cyanobacteria y una disminución de Chlorophyta y Bacillariophyta en todos los tratamientos con herbicida. Este efecto fue mayor con la adición de Roundup Max® y Glifosato II Atanor®, y fue menor con glifosato grado técnico, sugiriendo que los aditivos en los formulados comerciales pueden intensificar los efectos del glifosato.

Texto completo:

PDF (English)


American Publication Health Association. 2005. Standard methods for the examination of water and wastewater. 21st edition. Centennial Edition. APHA, American Water Works Association, Water Environmental Federation, Washington, DC, USA.

Annett, R., H. R. Habibi, and A. Hontela. 2014. Impact of glyphosate and glyphosate-based herbicides on the freshwater environment. J Appl Toxicol 34:458-479.

Aparicio, V., E. De Gerónimo, K. Hernández Guijarro, D. Pérez, R. Portocarrero, and C. Vidal. 2015. Los plaguicidas agregados al suelo y su destino en el ambiente. Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina.

Bischoff, H. W., and H. C. Bold. 1963. Phycological Studies IV. Some Soil Algae from Enchanted Rock and Related Algal Species. Univ. Texas Publ. Nº 6318, USA.

Carter, A. 2000. How pesticides get into water - and proposed reduction measures. Pesticide Outlook 11:149-156.

Castro Berman, M., D. J. G. Marino, M. V. Quiroga, and H. Zagarese. 2018. Occurrence and levels of glyphosate and AMPA in shallow lakes from the Pampean and Patagonian regions of Argentina. Chemosphere 200:513-522.

Duke, S. O. 1988. Glyphosate. Pp. 1-58 in P. C. Kearney and D. D. Kaufman (eds.) Herbicides: Chemistry, Degradation and Mode of Action. Marcel Dekker, New York, USA.

Forlani, G., M. Pavan, M. Gramek, P. Kafarski, and J. Lipok. 2008. Biochemical bases for a widespread tolerance of cyanobacteria to the phosphonate herbicide glyphosate. Plant Cell Physiol 49:443-456.

Gattás, F., A. Vinocur, M. Graziano, M. dos Santos Afonso, H. Pizarro, and D. Cataldo. 2016. Differential impact of Limnoperna fortunei-herbicide interaction between Roundup Max® and glyphosate on freshwater microscopic communities. Environ Sci Pollut Res 23:18869-18882.

Holtby, L. B., and S. J. Baillie. 1989. Effects of the herbicide Roundup (glyphosate) on periphyton in Carnation Creek, British Columbia. Pp. 224-231 in P. E. Reynolds (ed.). Proceedings of the Carnation Creek Herbicide Workshop, Forestry Canada, Canada.

Jeffrey, S. W., and G. F. Humphrey. 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanz 167:191-194.

Knudson, C. 2015. glmm: generalized linear mixed models via Monte Carlo likelihood approximation. R package version 1(2).

Krogh, K. A., B. Halling-Sørensen, B. B. Mogensen, and K. V. Vejrup. 2003. Environmental properties and effects of nonionic surfactant adjuvants in pesticides: a review. Chemosphere 50:871-901.

Lipok, J., T. Owsiak, P. Młynarz, G. Forlani, and P. Kafarski. 2007. Phosphorus NMR as a tool to study mineralization of organophosphonates-the ability of Spirulina spp. to degrade glyphosate. Enzyme Microb Technol 41:286-291.

Lipok, J., H. Studnik, and S. Gruyaert. 2010. The toxicity of Roundup Max® 360 SL formulation and its main constituents: glyphosate and isopropylamine towards non-target water photoautotrophs. Ecotoxicol Environ Saf 73:1681-1688.

Lowe, R. L., and Y. Pan. 1996. Benthic algal communities as biological monitors. Pp. 705-740 in R. J. Stevenson, M. L. Bothwell, and R. L. Lowe (eds.). Algal ecology. Freshwater benthic ecosystems. Academic Press, San Diego, California, USA.

Magbanua, F. S., C. R. Townsend, K. J. Hageman, K. Lange, G. Lear, G. D. Lewis, and C. D. Matthaei. 2013. Understanding the combined influence of fine sediment and glyphosate herbicide on stream periphyton communities. Water Res 47:5110-5120.

Pérez, G. L., A. Torremorell, P. Mugni, P. Rodríguez, M. S. Vera, M. Do Nascimento, L. Allende, J. Bustingorry, R. Escaray, M. Ferraro, I. Izaguirre, H. Pizarro, C. Bonetto, D. P. Morris, and H. Zagarese. 2007. Effects of the herbicide Roundup on freshwater microbial communities: a mesocosm study. Ecol Appl 17:2310-2322.

Pérez, G.L., M. S. Vera, and L. A. Miranda. 2011. Effects of herbicide glyphosate and glyphosate-based formulations on aquatic ecosystems. Pp. 343-368 in A. Kortekamp (ed.). Herbicides and environment. In Tech Open, Rijeka, Croatia.

Peruzzo, P. J., A. A. Porta, and A. E. Ronco. 2008. Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina. Environ Pollut 156:61-66.

Peterson, H. G., G. Boutin, P. A. Martin, K. E. Freemark, N. J. Ruecker, and M. J. Moody. 1994. Aquatic phytotoxicity of 23 pesticides applied at expected environmental concentrations. Aquat Toxicol 28:275-292.

Pizarro, H., M. S. Vera, A. Vinocur, G. Pérez, M. Ferraro, R. J. Menéndez Helman, and M. dos Santos Afonso. 2016. Glyphosate input modifies microbial community structure in clear and turbid freshwater systems. Environ Sci Pollut Res 23:5143-5153.

Powell, H. A., N. W. Kerby, and P. Rowell. 1991. Natural tolerance of cyanobacteria to the herbicide glyphosate. New Phytol 119:421-426.

R Development Core Team. 2014. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.

Rohr, J. R., J. L. Kerby, and A. Sih. 2006. Community ecology as a framework for predicting contaminant effects. Trends Ecol Evol 21:606-613.

Ronco, A. E., P. Carriquiriborde, G. S. Natale, M. L. Martin, H. Mugni, and C. Bonetto. 2008. Integrated approach for the assessment of biotech soybean pesticides impact on low order stream ecosystems of the Pampasic region. Pp. 209-239 in J. Chen and C. Guo (eds.). Ecosystem Ecology Research Trends. Nova Science Publishers, New York, USA.

Sabater, S., and W. Admiral. 2005. Periphyton as biological indicators in managed aquatic ecosystems. Pp. 159-78 in M. E. Azim, M. C. J. Verdegem, A. A. van Dam, and M. C. M. Beveridge (eds.). Periphyton: Ecology, exploitation and management. CABI Publishing, London, UK.

Sáenz, M. E., and W. D. Di Marzio. 2009. Ecotoxicidad del herbicida glifosato sobre cuatro algas clorofitas dulceacuícolas. Limnetica 28:149-158.

Sáenz, M. E., W. D. Di Marzio, J. L. Alberdi, and M. C. Tortorelli. 1997. Effects of technical grade and a commercial formulation of glyphosate on algal population growth. Bull Environ Contam Toxicol 59:638-644.

Torres, P. B., F. Chow, C. M. Furlan, and F. Mandelli. 2014. Standardization of a protocol to extract and analyze chlorophyll a and carotenoids in Gracilaria tenuistipitata Var. Liui. Zhang and Xia (Rhodophyta). Braz J Oceanogr 62:57-63.

Tsui, M. T. K., and L. M. Chu. 2003. Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors. Chemosphere 52:1189-1197.

Utermöhl, H. 1958. Zur vervollkommnung der quantitativen Phytoplankton Methodik. MIH Verh Int Ver Limnol 9:1-38.

Venrick, E. L. 1978. How many cells to count? Pp. 167-180 in A. Sournia (ed.). Phytoplankton manual. UNESCO, París, France.

Vera, M. S., L. Lagomarsino, M. Sylvester, G. L. Pérez, P. Rodríguez, H. Mugni, R. Sinistro, M. Ferraro, C. Bonetto, H. Zagarese, and H. Pizarro. 2010. New evidences of Roundup Max® (glyphosate formulation) impact on the periphyton community and the water quality of freshwater ecosystems. Ecotoxicology 19:713-719.

Vera, M. S., E. Di Fiori, L. Lagomarsino, R. Sinistro, R. Escaray, M. M. Iummato, A. Juárez, M. C. Ríos de Molina, G. Tell, and H. Pizarro. 2012. Direct and indirect effects of the glyphosate formulation Glifosato Atanor® on freshwater microbial communities. Ecotoxicology 21:1805-1816.

Vera, M. S., A. B. Juárez, and H. N. Pizarro. 2014. Comparative effects of technical-grade and a commercial formulation of glyphosate on the pigment content of periphytic algae. Bulletin of Environmental Contamination and Toxicology 93:399-404.

Wood, R. J., S. M. Mitrovic, R. P. Lima, and B. J. Kefford. 2016. How benthic diatoms within natural communities respond to eight common herbicides with different modes of action. ‎Sci Total Environ 557-558:636-643.

Enlaces refback

  • No hay ningún enlace refback.

ISSN en línea: 0327-5477; impresa 1667-782X (español); 1667-7838 (inglés)