The use of chronic bioassays in Daphnia magna for the environmental evaluation of an urban stream in Tierra del Fuego (Argentina)
DOI:
https://doi.org/10.25260/EA.21.31.2.0.1241Keywords:
ecotoxicity, urban impact, microcrustaceans, individual biomass, LC50Abstract
The development of urbanization has a negative impact on aquatic environments, making them the final receptors for a variety of anthropic substances, many of which lead to eutrophication. In Ushuaia (Tierra del Fuego), the Arroyo Buena Esperanza (ABE) has been subjected to these impacts. Its water quality decreases as the stream crosses the urban zone and discharges into Bahía Encerrada (BE). However, it is unknown whether its waters are toxic to the biota. We evaluated the water quality of the ABE all along its path through the urban zone and at its mouth in the BE through the effect on survival (48 hours) and growth (21 days) of Daphnia magna. Acute and chronic bioassays were conducted with D. magna neonates exposed to waters from four sites of the ABE: one with no urban impact, one with intermediate impact, and two with high impact. Water quality decreased as the stream crosses the urban zone, finding high values of ammonia, phosphates, particulate organic matter, total suspended solids and coliform bacteria; caused by the contribution of untreated sewage effluents. The survival of D. magna at 48 hours was close to 100%, which indicates the absence of acute toxicity of the water. The individual biomass varied according to the site and the time tested, increasing gradually but not evenly between sites. The organisms exposed to the most impacted sites presented higher biomass and growth rate in comparison to the sites with the least impact. This is linked to the poorer water quality of the impacted sites. The growth of D. magna was an environmental diagnostic tool with high applicability for the detection of sublethal effects of the waters of Fuegian streams, which adequately complements the environmental monitoring.
References
Abascal, L. 2016. Estudio de factibilidad para el saneamiento y la recuperación ambiental del frente marítimo y espacio acuático de la Bahía Encerrada. Informe Final Consolidado. Municipalidad de Ushuaia. Tierra del Fuego, Argentina.
Alves, A. C. B., and J. Silvano. 2006. Avaliação da sensibilidade de Daphnia magna Straus, 1820 (Cladócera, Crustácea) ao dicromato de potássio. Revista do Instituto Adolfo Lutz (Impresso) 65(1):59-61.
Amin, O., L. Comoglio, C. Spetter, C. Duarte, R. Asteasuain, R. H. Freije, and J. Marcovecchio. 2011. Assessment of land influence on a high-latitude marine coastal system: Tierra del Fuego, southernmost Argentina. Environmental Monitoring and Assessment 175(1-4):63-73. https://doi.org/10.1007/s10661-010-1493-5.
Angerville, R., Y. Perrodin, C. Bazin, and E. Emmanuel. 2013. Evaluation of ecotoxicological risks related to the discharge of combined sewer overflows (CSOs) in a periurban river. International Journal of Environmental Research and Public Health 10:2670-2687. https://doi.org/10.3390/ijerph10072670.
APHA- AWWA- WEF. 2017. Standard Methods for the Examination of Water and Wastewater. Washington.
Baun, A., A. M. Christensen, and F. Nakajima. 2003. Ecotoxic effects of wet weather discharges in an urban stream. Diffuse Pollution Conference Dublin.
Becouze-Lareure, C., L. Thiebaud, C. Bazin, P. Namour, P. Breil, and Y. Perrodin. 2016. Dynamics of toxicity within different compartments of a peri-urban river subject to combined sewer overflow discharges. Science of the Total Environment 539:503-514. https://doi.org/10.1016/j.scitotenv.2015.08.128.
Biancalana, F., and A. I. Torres. 2011. Variations of mesozooplankton composition in a eutrophicated semi-enclosed system (Encerrada Bay, Tierra del Fuego, Argentina). Brazilian Journal of Oceanography 59(2):195-199. https://doi.org/10.1590/S1679-87592011000200008.
Castillo Morales, G. 2004. Ensayos toxicológicos y métodos de evaluación de calidad de aguas. Estandarización, intercalibración, resultados y aplicaciones. First edition. IMTA, México.
Castro-Mejía, J., J. A. Ocampo-Cervantes, G. Castro-Mejía, I. Cruz-Cruz, M. C. Monroy-Dosta, and D. Becerril-Cortes. 2016. Laboratory production of Daphnia magna (Straus 1820) fed with microalgae and active dry yeast. Journal of Entomology and Zoology Studies 4(2):548-553.
Chalmers, A. T., P. C. Van Metre, and E. Callender. 2007. The chemical response of particle-associated contaminants in aquatic sediments to urbanization in New England, U.S.A. Journal of Contaminant Hydrology 9:4-25. https://doi.org/10.1016/j.jconhyd.2006.08.007.
Chantanachat, S., and H. C. Bold. 1962. Phycological Studies II. Some algae from arid soils. University of Texas Publication N° 6218. Austin, Texas.
Christensen, A. M., F. Nakajima, and A. Baun. 2006. Toxicity of water and sediment in a small urban river (Store Vejleå, Denmark). Environmental Pollution 144(2):621-625. https://doi.org/10.1016/j.envpol.2006.01.032.
Damásio, J., R. Tauler, E. Teixidó, M. Rieradevall, N. Prat, M. C. Riva, A. M. N. M. Soares, and C. Barata. 2008. Combined use of Daphnia magna in situ bioassays, biomarkers and biological indices to diagnose and identify environmental pressures on invertebrate communities in two Mediterranean urbanized and industrialized rivers (NE Spain). Aquatic Toxicology 87(4):310-320. https://doi.org/10.1016/j.aquatox.2008.02.016.
Díaz Báez, M., Y. Pica Granados, and A. Ronco. 2004. Ensayo de toxicidad aguda con el cladócero Daphnia magna. Pp. 52 -63 in G. Castillo Morales (ed.). Ensayos toxicológicos y métodos de evaluación de calidad de aguas. IMTA, México.
Diodato S., L. Comoglio, C. Camilión, and O. Amin. 2012. Responses of the resident rocky crab (Halicarcinus planatus, Decapoda) to natural stressors and effluent discharges in Ushuaia Bay, Tierra del Fuego, Argentina. Journal of Experimental Marine Biology and Ecology 436-437:11-18. https://doi.org/10.1016/j.jembe.2012.08.011.
Diodato, S. 2013. Respuestas de especies locales a procesos de eutrofización en la zona costera próxima a la ciudad de Ushuaia, Tierra del Fuego. Tesis Doctoral. Universidad Nacional del Sur, Bahía Blanca. Argentina. Pp. 221.
Diodato, S., L. Comoglio, A. Moretto, and J. Marcovecchio. 2018. Dinámica e impacto de la eutrofización por aportes urbanos en las cuencas hídricas y zona costera de la ciudad de Ushuaia, Tierra del Fuego. Pp. 468-475 in Abraham, E., R. Quintana and G. Mataloni (eds.). Aguas + Humedales, Serie Futuros. UNSAM EDITA, Buenos Aires.
Diodato, S., G. González Garraza, R. Mansilla, A. Moretto, J. Escobar, M. Méndez‑López, A. Gómez‑Armesto, J. Marcovecchio, and J. Nóvoa-Muñoz. 2020. Quality changes of fluvial sediments impacted by urban effluents in Ushuaia, Tierra del Fuego, southernmost Patagonia. Environmental Earth Sciences 79:481. https://doi.org/10.1007/s12665-020-09236-4.
Dirección General De Recursos Hídricos. 2013. Informe de calidad de aguas de la ciudad de Ushuaia, octubre de 2013. Informe Técnico. Secretaría de Ambiente, Desarrollo Sostenible y Cambio Climático. Tierra del Fuego, Argentina.
Dirección General De Recursos Hídricos. 2015. Informe de monitoreo de calidad de agua - Plan Hidroambiental de la ciudad de Ushuaia sector este. Octubre 2015. Secretaría de Ambiente, Desarrollo Sostenible y Cambio Climático. Tierra del Fuego, Argentina.
Dumont, H. J., I. Van De Velde, and S. Dumont. 1975. The dry weight estimate of biomass in a selection of Cladocera, Copepoda and Rotifera from the plankton, periphyton and benthos of continental waters. Oecologia 19(1):75-97. https://doi.org/10.1007/BF00377592.
Ebele A. J., M. A. E. Abdallah, and S. Harrad. 2017. Pharmaceuticals and personal care products (PPCPs) in the freshwater aquatic environment. Emerging Contaminants 3:1-16. https://doi.org/10.1016/j.emcon.2016.12.004.
Ferrari, L. 2006. Generalidades sobre ecotoxicología. Pp. 393-421 in Carballo M. and M. Mudry (eds.). Genética Toxicológica. Editorial Los Cuatro Vientos, Buenos Aires, Argentina.
Giraudo, M., M. Douville, and M. Houde. 2015. Chronic toxicity evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna transcriptomic response. Chemosphere 132:159-165. https://doi.org/10.1016/j.chemosphere.2015.03.028.
Giraudo, M., T. L. Colson, M. Pilote, C. Gagnon, P. Gagnon, and M. Houde. 2019. A major release of urban untreated wastewaters in the St. Lawrence River (Quebec, Canada) altered growth, reproduction, and redox status in experimentally exposed Daphnia magna. Ecotoxicology 28(7):843-851. https://doi.org/10.1007/s10646-019-02084-4.
Göbel, P., C. Dierkes, and W. G. Coldewey. 2007. Storm water runoff concentration matrix for urban areas. Journal of Contaminant Hydrology 91:26-42. https://doi.org/10.1016/j.jconhyd.2006.08.008.
Gopi, R. A., S. Ayyappan, G. Chandrasehar, V. Krishna, and A. Goparaju. 2012. Effect of potassium dichromate on the survival and reproduction of Daphnia magna. Bulletin of Environment, Pharmacology and Life Sciences 1(7):89-94. https://doi.org/10.5402/2012/247072.
Granitto, M., S. Diodato, and P. Rodríguez. 2021. Water quality index including periphyton chlorophyll-a in forested urban watersheds from Tierra del Fuego (Argentina). Ecological Indicators 126:107614. https://doi.org/10.1016/j.ecolind.2021.107614
Iturraspe, R. 2007. Report on basin response for Argentina. Deliverable D18 EPIC ORCE. Tierra del Fuego, Argentina.
Kosmala, A., S. Charvet, M. C. Roger, and B. Faessel. 1999. Impact assessment of a wastewater treatment plant effluent using instream invertebrates and the Ceriodaphnia dubia chronic toxicity test. Water Research 33(1):266-278. https://doi.org/10.1016/S0043-1354(98)00176-6.
Le Moal, M., C. Gascuel-Odoux, A. Ménesguen, Y. Souchon, C. Étrillard, A. Levain, F. Moatar, A. Pannard, P. Souchu, A. Lefebvre, and G. Pinay. 2019. Eutrophication: A new wine in an old bottle? Science of the Total Environment 651:1-11. https://doi.org/10.1016/j.scitotenv.2018.09.139.
Marcogliese, D. J., C. Blaise, D. Cyr, Y. De Lafontaine, M. Fournier, F. Gagné, C. Gagnon, and C. Hudon. 2015. Effects of a major municipal effluent on the St. Lawrence River: A case study. Ambio 44(4):257-274. https://doi.org/10.1007/s13280-014-0577-9.
Martin-Creuzburg, D., and E. Von Elert. 2009. Good food versus bad food: the role of sterols and polyunsaturated fatty acids in determining growth and reproduction of Daphnia magna. Aquatic Ecology 43(4):943-950. https://doi.org/10.1007/s10452-009-9239-6.
Martínez-Jerónimo, F., J. Rodríguez-Estrada, and L. Martínez-Jerónimo. 2008. Daphnia exilis Herrick, 1895 (Crustacea: Cladocera): Una especie zooplanctónica potencialmente utilizable como organismo de prueba en bioensayos de toxicidad aguda en ambientes tropicales y subtropicales. Revista Internacional de Contaminación Ambiental 24(4):153-159.
OECD. 2008. Guidelines for Testing of Chemicals. Daphnia magna Reproduction Test OECD 211. OECD Publishing, Paris.
Rörig, L., J. Tundisi, C. Schettini, J. Pereira-Filho, J. Menezes, T. Almeida, S. Urban, C. Radetski, R. Sperb, C. Stramosk, R. Macedo, M. Castro-Silva, and J. Pérez. 2007. From a water resource to a point pollution source: the daily journey of a coastal urban stream. Brazilian Journal of Biology 67(4):597-609. https://doi.org/10.1590/S1519-69842007000400003.
Scholes, L., A. Baun, M. Seidl, E. Eriksson, M. Revitt, and J. Mouchel. 2007. Assessment of storm water ecotoxicity using a battery of biotests. Pp. 399-410 in Morrison G. and S. Rauch (eds.). Highway and Urban Environment: Proceedings of the 8th Highway and Urban Environment Symposium. Springer. https://doi.org/10.1007/978-1-4020-6010-6_35.
Serra, T., M. Soler, N. Pous, and J. Colomer. 2019. Daphnia magna filtration, swimming and mortality under ammonium, nitrite, nitrate and phosphate. Science of the Total Environment 656:331-337. https://doi.org/10.1016/j.scitotenv.2018.11.382.
Steinkey, D., E. Lari, S. Woodman, K. Luong, C. Wong, and G. Pyle. 2018. Effects of gemfibrozil on the growth, reproduction, and energy stores of Daphnia magna in the presence of varying food concentrations. Chemosphere 192:75-80. https://doi.org/10.1016/j.chemosphere.2017.10.124.
Sterner, R. W., D. D. Hagemeier, W. L. Smith, and R. F. Smith. 1993. Phytoplankton nutrient limitation and food quality for Daphnia. Limnol. Oceanogr. 38(4):857-871. https://doi.org/10.4319/lo.1993.38.4.0857.
Strauss, H. E. 1820. Memoire sur les Daphnia de la clase des Crustaces (seconde partie). Memoires du Musee Royale d'Histoire Naturelle de Belgique 6:149-162.
Strickland, J., and T. Parsons. 1972. A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada 167.
Tamura, I., Y. Yasuda, K. I. Kagota, S. Yoneda, N. Nakada, V. Kumar, Y. Kameda, K. Kimura, N. Tatarazako, and H. Yamamoto. 2017. Contribution of pharmaceuticals and personal care products (PPCPs) to whole toxicity of water samples collected in effluent-dominated urban streams. Ecotoxicology and Environmental Safety 144:338-350. https://doi.org/10.1016/j.ecoenv.2017.06.032.
Ten Berge, W. F. 1978. Breeding Daphnia magna. Hydrobiologia 59(2):121-123. https://doi.org/10.1007/BF00020772.
Torres, A., M. Gil, O. Amin, and J. Esteves. 2009. Environmental characterization of an eutrophicated semi-enclosed system: nutrient budget (Encerrada Bay, Tierra del Fuego Island, Patagonia, Argentina). Water, Air, and Soil Pollution 204:259-270. https://doi.org/10.1007/s11270-009-0042-8.
USEPA. 2002. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. U.S. Environmental Protection Agency. Washington.
Urciuolo, A., and R. Iturraspe. 2005. Ordenamiento hídrico de las cuencas de fuentes aptas para provisión de agua potable a la ciudad de Ushuaia. Proccedings of the 20th Congreso Nacional del Agua. May 9-14. 2005. Mendoza, Argentina.
Van Leeuwen, J., J. Awad, B. Myers, and D. Pezzaniti. 2019. Introduction to Urban Stormwater: A Global Perspective. Pp. 1- 28 in Jegatheesan V., A. Goonetilleke, J. van Leeuwen, J. Kandasamy, D. Warner, B. Myers, M. Bhuiyan, K. Spence and G. Parker (eds.). Urban Stormwater and Flood Management. Applied Environmental Science and Engineering for a Sustainable Future. Springer Nature Switzerland.
Verma, Y. 2008. Toxicity assessment of pulp-paper mill effluents employing Daphnia bioassay. Japanese Journal of Environmental Toxicology 11(2):151-156.
Zagarola, J., G. Martínez Pastur, M. Lopez, and C. Anderson. 2017. Assessing the effects of urbanization on streams in Tierra del Fuego. Ecología Austral 27:45-54. https://doi.org/10.25260/EA.17.27.1.0.417.
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